Microsatellite markers or SSR (Simple Sequence Repeats) and RAPD have proved to be an excellent tool for cultivars identification, pedigree analysis and the evaluation of genetic distance among organisms. Soybean cultivars have been characterized mainly by morphological and biochemical traits. However‘ these traits have not been sufficient to characterize the large number of cultivars eligible to receive protection. In order to define new soybean cuitivars markers the RAPD and SSR markers were used to distinguish the morphologically similar groups. A dendrogram based on the RAPD and SSR profiles showed a good agreement with the cultivars pedigree information.