The aim of this investigation was to study the effect of propolis phenolic extract on hyperlipaemia in rats. The propolis phenolic extract was analyzed by high performance liquid chromatography (HPLC) to 74 constituents. The major known identified phenolic compounds of propolis were caffeic acid, salicylic acid, cinnamic acid, ferulic acid and kaempferol. Propolis extract caused significantly decrease in total lipids, total cholesterol, triglycerides and LDL- cholesterol contents in serum as well as in liver. On the contrary, HDL- cholesterol was increased in normai and hypercholesterolemic rats compared with the control groups. Furthermore, gain of body weight, food intake and feed efficiency ratio were carried out. From the data, it could be deduced that the propolis phenolic constituents had a remarkable am&iorative effect against hyperlipaemia.