Two field experiments were carried out during two seasons in Agricultural Experimental Station of Desert Research Center at Wadi Sudr, to study the effects of salinity levels ((3607 and 4602 ppm) and cycocel application (00, 250, 500 and 1000 ppm) on physical, chemical properties and fatty acids composition of oil extracted from seeds of two canola cultivars (Pactol and Serw4) grown under salt-affected calcareous soils. The data indicated clearly that, acid, peroxide and saponification values were increased in oil of Pactol and serw4 cultivars under high level of salinity at O and 6 month of storage. While, the iodine value was significantly decreased in oil of two cultivars under high level of salinity at O and 6 month of storage. Acid value was increased by spraying dose of 500 and 1000 ppm CCC for Pactol and Serw4 cultivars, respectively. Also, Pactol and Serw4 cultivars gave the maximum values of peroxide value by spraying dose of 500 ppm CCC. While iodine value took the reverse effect for two canola cultivars alter treatment with 500 ppm CCC. After 6 month of storage at room temperature, acid and peroxide values for Pactol cultivar were increased by spraying dose of 500 ppm CCC. While, acid and peroxide values for serw4 cultivar were decreased by spraying of 250 and 1000 ppm CCC, respectively. In this regard, the spraying of CCC tended to increase iodine value for two canola cultivars. Caproic, lauric, myristic, palmitic, stearic, oleic, linoleic, linolenic, arachidic, Cis-11-eicosenoic and behenic acids were detected in most samples of canola o1 under study. The predominant saturated fatty acid in two canola cultivars (Pactol and Serw4) is palmitic acid. The major constituents of unsaturated fatty acids ¡n oil extracted from two canola cultivais were oleic, linoleic and linolenic acids. Concerning the erucc acid content (which s the main limiting factor for canola oil use) in oil of two canola cultivars, it was increased under high level of salinity. Spraying of Cycocel under low level of safinity tended to increase the erucic acid in oil of serw4 cultivar, when applied at rate 1000 ppm CCC. Under high level of salinity, erucic acid for Pactof was increased with increasing cycocel levels. While, Serw4 cultivar took the reverse effect for such content, when applied at rates 250 and 1000 ppm CCC.