Two field experiments were conducted at El-Serw Agricultural Research Station, Damietta Governorate during the two seasons 2001/2002 and 2002/2003 to investigate the influence of mineral nitrogen fertilizer levels and biofertilizers, Serialin  and/or Phosphorien, and accordingly their interactions on wheat growth, yield and its attributes as well as N and p uptake of wheat grain and straw and also grain protein content. A split plot design with four replicates was used. The obtained results could be summarized as follows: -        Raising meniral nitrogen fertilizer level from 45 to 60 and 75 kg N/fed. resulted in  significant increases in plant height, number of tillers/plant, spike length, 1000-grain weight, grain and straw yields/fed. and protein content of grain. Also N and P uptake of grain and straw were significantly increased in both seasons. -        Inoculation of wheat grain with biofertilizers, Serialin and/or Phosphorein, resulted in marked increase in plant height, spike length, 1000 grain weight, grain and straw yields/fed., protein content of grain, N and p uptake of both grain and straw, compared with that uninoculated treatment in both seasons. -        Dual inoculation with Serialin and Phosphorein gave the highest values, while the lowest values were recorded with the uninoculated treatment. -        The interaction between mineral nitrogen levels and biofertilization had a significant effect on grain and straw yields/fed. In both seasons, N uptake of grain and straw in the second season. Also, P uptake of grain and straw in the second season. -        Although, the maximum wheat yield and its components were recorded with the dual inoculation with Serialin and Phosphorein at the highest N rate (75 kg N/fed.), rate of 60 kg N/fed. with the dual inoculation was most compatible, since it has the highest wheat yield over uninoculation control. Finally, it could be concluded that inoculation wheat grains with dual inoculants Serialin and Phosphorein with using 80% of recommended dose (i.e. 60 kg N/fed.) is the recommended treatment for maximizing wheat yield and net return as well as reducing mineral fertilization and soil pollution.