The response and improvement of root colonization, yield, growth and N- uptake of wheat grown in salt affected soil (with pH value in the alkaline side) were studied. In a field experiment conducted and carried out during the season of 20052006, the biofertilizer inoculation (Azotobacter chroococcum andfor Azospin'iium brasiiense) in combined with different rates of N—fertilizer (ammonium nitrate at 0, 20, 40, 60 and 30 Kg. N fed") were applied. The treatments were arranged in split plot design with three replicates. The results showed that there is an increment in Azospinlfum count in wheat rhizosphere soil with single inoculation of Azospiriiium, which gave highest number of Azospirilfum after 60 days of planting, then reversible results were obtained at the end of cultivation period (120 days), where, the dual inoculation gave highest number of Azospirilium compared with the other treatments. Also, the inoculation of wheat grains with Azolobacter, led to gradual increases in the counts of Azotobacter in wheat rhizosphere soil up to 90 days, then, decreased at the end of cultivation period. All inoculated treatments gave higher counts of Azotobacler compared with the uninoculated treatment. Generally, the total Nz—fixers and total bacterial counts increased greatly in wheat rhizosphere soil in inoculation treatments compared with uninoculated treatments. The results showed significant increases in plant dry weight, grain and straw yields as well as nitrogen uptake by wheat plants either by increasing the rate of mineral nitrogen or with inoculation by tested Ng-fixers. In addition, the dual inoculation with Azotobacler chroococcum and Azospinlium brasilense performed significantly greater followed by single inoculation with Azolobacter or Azospin'lium. At any level of N-fertilizer, the inoculated treatments gave much higher straw and grain yields than the uninoculated one. Finally, it could be concluded that in salt affected soil, the amount of mineral N fertilizer could be reduced by using biofertilizers, which in turn increases soil fertility as well as, minimizes the production cost and environmental pollution, which can occur by the excess use of chemical fertilizers.