Laboratory experiments were executed in batch-operating digesters at mesophilic conditions (40 ºC) to optimize biogas production from poultry droppings at different adding times of agrotain (Urease enzyme inhibitor). A comparative analysis of biogas yield and chemical composition of the influent and effluent slurries as organic fertilizer for the different treatments were also carried out. Biogas composition (Methane, carbon dioxide and hydrogen sulfide) of biogas were found. Influent and effluent enumeration of total fecal califorms and Salmonella was evaluated. Biogas production from treatment 3 was 37.7, 15.2 and 44.9 % higher than that of treatments 1, 2 and 4, respectively. The fermentation process efficiency for the four different treatments was 47.8, 64.0, 72.8 and 45.7%, respectively. Absence of total and fecal coliform and Salmonella of all effluent slurries of the different treatments was noticed. The digested treatments under study have the potential of being soil conditioners.