In the present study, seeds from local cultivar of fennel were germinated on Murashige and Skoog medium (MS) without plant growth regulators. Different types of explants from the growing seedling such as cotyledonal leaves, hypocotyls, epicotyls and roots were cultured on MS medium. The medium contained different concentrations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) either alone or with kinetin. Differential responses in the essential oil were observed in the induction and development of callus. The major components of essential oils included estragole, trans-anethole, limonene and fenchone were proposed to be used in chemical classification on callus.