Gel electrophoresis of protein has become a standard and powerful research tool for application in biochemical sciences. The aim of this study was to develop biochemical genetic marker such as protein electrophoretic profiles which resulted from gene expression to discover the relationship between the strain genotypes and the environmental effect under normal and salinity conditions. The results showed that appearance, disappearance and intensity variation of banding patterns in grains protein under saline treatments in studied two genotypes. From SDS- PAGE showed the represented two bands where Rm was 0.69 and 0.89 which were appeared only under saline treatment in S₂. In addition represented only one band where Rm was 0.89 which were appeared only under environmental saline treatment in S₃. These results clearly suggested the presence of an association between salt tolerance and the presence of these bands having stability against the environmental salinity. These results revealed that some bands could be considered as a salt shock protein band which appeared after salt treatments. Finally, the protein electrophoresis system was a reliable system for discriminating strains under variations of environments conditions.