A pot experiment was carried out to evaluate the growth and N-use efficiency of three grapevine varieties (Crimson Seedless, Superior and Thompson seedless). Nitrogen was added at five rates: 0, 10, 40, 80 and 120 mg N kg^-1 as ammonium nitrate (33.5 % N). Plant samples were collected after two and six weeks from the ending of nitrogen application treatments. The obtained results showed that, irrespective of varieties, increasing N-rates increased root, shoot growth and N-content but decreased N-use efficiency. However, Crimson recorded the highest values of all root, shoot growth parameters, N-content and N-use efficiency compared with the other two varieties.  On the other hand, the interaction effect of N-rates and grapevine varieties showed that Crimson had the highest root length, root fineness values, root and shoot dry weight, shoot length, leaf area, N-content. Crimson recorded the highest nitrogen use efficiency at all N-rates compared with the two other grapevine varieties. Based on the result of the present study, Crimson variety can produce well under low N level and responded well to N-application.