This study designed to investigate the role of high protein diet, vitamin C and vitamin E as radioprotectors in rats followed exposure to 7 Gy whole body gamma irradiation.  120 male albino rats were divided into six equal groups of 20 rats each: control, irradiated, high protein (P), vitamin C, vitamin E and combination C+E+P supplemented groups.  The variation in activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), urea and creatinine concentrations were estimated for all groups.  Serum apoptosis detection was carried out according to the Enzyme-Linked ImmunoSorbent Assay technique (ELISA). In the irradiated group, an increase in serum ALT and AST activities, urea and creatinine concentrations and apoptosis rate were observed, whereas in almost irradiation treated groups, the reverse was occurred. Based on these biochemical observations, it was concluded that high protein, vitamin C and vitamin E treatment exerts a protective effect against irradiation damage, especially in serum AST, creatinine and apoptosis.