The effect of daily oral administration of Carbamate (  Methomyl) ,at   0.2 , 2 and 5 mg/kg b.w  and  neonicotiniod ( Imidacloprid ),  at  0.5 ,1 and 5 mg/kg b.w    insecticides  on male  albino rats for 28 successive days, followed by 15 days recovery were studied . The most important biochemical  parameters of the serum ; alanine  aminotranseferase ( ALT) , aspartate aminotraseferase (AST) ,alkaline phosphatase activities ( ALP )  , total protein , protein profile (albumin and  globulin)  , urea , creatinine were   investigated .   The activities  of AST ,ALT were significantly  increased  at all tested times in cumulatively dose related manner .No significant changes were  observed in alkaline phosphatase enzyme activity compared with the control.   On the other hand serum total protein ,  Protein Profile (albumin and  globulin ) recorded  highly significant decrease in all treatments with Methomyl and imidacloprid  . A highly significant increases  in the blood urea and creatinine with two tested insecticides in the following order ,methomyl and imidacloprid. after treatments 14 days were observed . The levels were decreased  during the 21, 28 and 45 days.