The present study was designed to investigate the effect of feeding on diet containing 5% of raw fenugreek flour (RFF) and soaked fenugreek flour (SFF) or 5% and 10% of germinated fenugreek flour (GFF) on the blood glucose level, lipid profile and serum transaminase enzymes, as well as, body weight, food intake, food efficiency ratio (FER) and some organs weight in streptozotocin-induced diabetic rats. The obtained results could be summarized as follows:                 The supplementation of basal diet with fenugreek flour led to significant increase of final body weight, body weight gain (%), food intake, FER and organs weight of diabetic rats specially with 5 and 10% of GFF, while relative organs weight were decreased with 5% of SFF or 5 % and 10 % GFF compared with that recorded of diabetic control. In relation to blood glucose level, the addition of RFF to the basal diet reduced blood glucose level of diabetic rats but still in the range of diabetic > 248.4 mg/dl, while supplementation of basal diet with SFF resulted to a decrease in blood glucose level (231.9 mg/dl) but still near from the range of diabetic. On the other hand, GFF at two levels (5 and 10%) caused to more reduction of blood glucose level of diabetic rats, and the more effect was observed with 10% GFF (209.6 mg/dl). Streptozotocin-induced diabetic rats had a higher level of total cholesterol (TC), low density lipoprotein- cholesterol (LDL-C) and total cholesterol/high density lipoprotein- cholesterol (TC/HDL-C) and lower level of HDL-C comparing with normal rats. The addition of fenugreek seeds flour in all forms to basal diet improved the previous parameters and they became in the normal range (TC, 110.86-143.45; LDL-C, 47.10-93.47; TC/HDL-C, 1.74-2.87 and HDL-C, 49.89-63.76 mg/dl). The feeding of diabetic rats on diet containing 5% or 10% GFF decreased the liver content of total lipids, phospholipids, cholesterol and triglycerides (TG) to the level, where no significant difference was noticed with normal control. Also, the results revealed that, fecal output, fecal lipid and fecal cholesterol excretion were increased with supplementation of basal diet with raw, soaked or germinated fenugreek flour. This supplementation improved serum transaminase enzymes especially alanine-aminotransferase enzyme (AST). So, it can be recommended that, using fenugreek flour especially germinated fenugreek flour at the level of 10 % had a pronounced effect in reducing blood glucose and cholesterol levels.