Assiut Governorate, Egypt, was divided using five transects across the Nile valley in the east-west direction in order to assess the various forms of Cu and Mn. Surface soil samples were collected from an average of six locations for each transect. Soil zinc and copper forms including soluble + exchangeable, carbonate-bound, Mn oxide-bound, organic-bound, poorly crystalline Fe oxide-bound and crystalline Fe oxide-bound forms of the studied samples were determined in a sequential extraction. The total contents of Zn and Cu in these samples were also determined.               Concentrations of soil Zn in these forms decreased in the order of: Residual > PCFeOx > CFeOx > O-bound > Carb. > Mn Ox > Exch.form. Levels of Zn in these respective forms represented 91.86, 3.81, 2.76, 0.59, 0.42, 0.37 and 0.22% of the total soil Zn.         The mean levels of soil Cu bound to the residual, crystalline Fe oxide, poorly crystalline Fe oxide, organic matter, Mn oxide, carbonate and exchangeable forms were 43.62 (82.69% of total Cu), 1.35(2.56% of total Cu), 5.06 (9.59% of total Cu), 0.94 (1.78% of total Cu), 0.33 (0.63% of total Cu), 0.82 (1.55% of total Cu) and 0.63 mg/kg (1.19% of total Cu), respectively. The total Zn content of these soils had an average value of 52.75 mg/kg.