Two field experiments were carried out at Manshaat Abdel-Rahman Dakahlia Governorate during winter seasons of 2004/2005 and 2005/2006 to study the effect of biofertilizer (Nitrobein), Chemical fertilizer as soluble (urea and ammonium nitrate) and slow release urea formaldehyde coated urea (UFCU) with foliar humic and amino acid on yield (grain and straw), uptake (N, P andK) and content of micronutrients (Fe, Zn, Mn and Cu) on wheat plants in clay soil. The experiment consists of 32 treatments which replicated 4 times. The experiment was factorial in complete randomized block design. The three studied effects of biofertilization, chemical N fertilizers (urea, AN and UFCU) and foliar of humic and amino acid as well as interaction between them exhibited a significantly positive effect and also: Inoculation of wheat grain by nitrobein as a biofertilizer under chemical N forms (Urea, AN and slow release UFCU) superposed chemical N fertilizers only. Used of slow release N fertilizer (UFCU) superiority chemical N fertilizer (urea and AN). The interactions of biofertilizer, chemical N and foliar humic and amino acids. The study under the present conditions recommended nitrobein as biofertilization with slow release (UFCU) at rate 75 kg N/fed and foliar humic and amino acids, where it can give high production of grain yield of wheat and can lower environmental pollution.