Three vegetable oils (virgin olive, crude canola, or linseed oils) were used as diet additives to compare their effects on lipid profile of blood serum of experimental rats in the presence of egg yolk as a source of cholesterol. Corn meal (1.6% fat) was used as control diet, the positive control diet composed of corn meal plus 9% egg yolk (about 0.15%cholesterol). The other three diets composed of positive control plus 14% olive, canola or linseed oils. Lipid parameters were determined in rat's blood serum after 21, 36 and 46 days from feeding . Obtained data revealed that both canola and linseed oils containing diets  significantly decreased total cholesterol (TC), triacylglycerol(TG) and low-density lipoprotein cholesterol (LDL-c) and increased high-density lipoprotein cholesterol (HDL-c)  levels in relation to positive control diet.  Data of olive oil containing diet showed a  significant  increase in TC, TG, and HDL-c at the end of nutrition experiment. Whereas no significant difference in LDL-c between positive control and olive oil containing diet.