During the two consecutive summer seasons of 2015 and 2016 at Faculty of Agric.; Damietta University, Damietta Governorate, Egypt, two pot experiments were conducted to examine the effect of phosphorus fertilization, organic fertilization and inoculation with Azotobacter as well as their interactions on nutrient uptake and yield of soybean plants (Glycine max L.). The experiment was performed using split-split plot design with 18 treatments in three replicates, three treatments of organic manure in form of chicken manure (without, 8 and 12 ton.fed^-1) as the main plot, three rates of phosphorus fertilization (0, 36 and 54 kgp < sub>2o₅.fed^-1) as subplot and all treatments were studied twice; once in the presence of Azotobacter strains inoculants and the other without it as sub-sub plot. Individual organic manure increased N, P and K uptake by the plant, yield and its component (100-seed, g/plant, seed yield, g/plant, pod length, cm and pods weight, g/plant) and chemical composition of seed (N, P, K, protein and total carbohydrates%). Obtained results declare that phosphorus fertilizer treatments at 54 kg P₂O₅.fed^-1 recorded the highest values of the mentioned parameters. Bio-fertilizers increased the traits especially in presence of Azotobacter comparing with the un-inoculated plant. So, the interaction between 12 ton.fed^-1 chicken manure and 54 kg.fed^-1 P₂O₅ in presence of Azotobacter was the best treatment to achieve the highest values of parameters under investigation during two seasons.