Two field experiments were carried out in two successive winter seasons of 2006/2007 and 2007/2008 to study the effect of bio-and mineral phosphorus fertilization on the yield and nutritional value of canola plant grown in calcareous soil at El -Areish Agricultural Research station,) ARC)  North Sinai Governorate. The main results were as the following: superphosphate and rock phosphate achieved a similar and insignificant effect on most measured yield characteristics, seed yield, N, P, K, oil, protein contents and oil yield in both growth seasons. Inoculation with either mycorrhiza or Bacillus megatherium alone or Bacillus megatherium + mycorrhiza were significantly resulted in actions on most canola tested parameters compared to the treatment of without inoculation. However, seed yield and P percentage did not respond significantly toward the inoculation with the investigated biofertilizers in first season. Generally the highest values obtained. for most characteristics of canola plants under investigation were achieved when the plants received rather rock phosphate or superphosphate mineral fertilizers together with mycorrhiza and/or Bacillus megatherium + mycorrhiza. While, there were insignificant differences due to their interaction on No. of branches / plant, seed yield and P percentage as well as canola plant height both in the first and second seasons.