In this work, the effect of two different cooling temperatures (T_c) on the permeate flux (F_p) and thermal efficiency (η) of air gap membrane distillation (AGMD) module was studied experimentally. All data were described statistically in terms of means, standard error for means (SE), standard deviation (SD) and coefficient of variation (C.V.). Data were subjected to the analyses of the variance (ANOVA) and multivariate analysis to test the significant effect of different feed and designs at pf) and flow rates (M_f). Experimental results revealed that the percentage of AGMD permeate flux was reduced by 4.71%, 4.98%, 7.51%, 1.84%, 2.71%, 8.20%, 2.25% and 4.42% at increasing cooling temperature from 10 to 20 ^oC, respectively, in case of flow rate (M_f) of 2.0 and 4.0 L/m (temperature T_f of 50 ^oC), 6.0 and 8.0 L/m (temperature T_f of 50, 60 and 70 ^oC) due to lowering the temperature and vapor pressure differences between the hot and the cold feed at both sides of the membrane (ΔT_cross=T₁-T₄). Regarding thermal efficiency (η), an opposite result was obtained whereas the thermal efficiency was improved significantly at increasing cooling temperature from 10 to 20 ^oC at all different operating conditions. The rate of increasing in thermal efficiency were around 1.26, 1.29, 1.15 and 1.27 times at flow rate M_f of 2 L/m and different temperature T_f of 50, 60, 70 and 80 ^oC, respectively.