Two field trials were conducted at El-Serw Agricultural Research Station, Dammietta governorate through summer season 2006 and 2007, to study the effect of organic matter as compost (20 m³.fed^-1), N-biofertilization (cyanobacteria Azospirillum, Bacillus inoculations and mix with previous inoculations) and mineral nitrogen applications (20, 40 and 60 kg-N.fed^-1) on rice grain and straw yield, N, P and K uptake and nitrogen use efficiency for rice crop (Oryza sativa), variety Giza 178. The results showed that rice grain and straw yield and N, P and K-uptake in grain and straw increasing with use of nitrogen fertilizer rates up to 60 kg N / fed, while decreasing values of nitrogen use efficiency for rice crop. As well as the results showed that blue-green algae, a mixture of bio-fertilizers, Azospirillum, and Bacillus inoculations, respectively gave the highest values of the previous parameters except the NUE, where the order of values was upward with the order of the previous inoculations. Also, the results showed that the use of organic matter as a compost gave the highest values of the previous parameters and lowest values of NUE for rice crop. Organic matter + Blue green algae  inoculation + 60 kg N.fed-1 gave high rice grain and straw yield and nutrients uptake and gave the lowest N-use efficiency. Also, applying organic matter + BGA or mix or Azospirillum inoculation could produce economic rice grain yield when it combined with third dose of the recommend mineral nitrogen (20 kg-N.fed^-1), and in the same time, this treatment gave high nitrogen use efficiency, and thus save on mineral nitrogen fertilization, which may lose when applying the full recommended dose, and conserve environment by reducing pollution hazards.