Effect of nitrogen (N) and potassium (K) fertilizers application on the uptake and availability of nitrogen and potassium was studied at the Farm of Rice Research and Training Center at Sakha Kafr El-sheikh during 2007 and 2008 seasons. The highest values of available NH₄-N in the soil were recorded at 30 DAT under 92 kg N fed^-1 with application full dose of potassium before transplanting (50 Kg potassium sulphate .fed^-1). Data showed also that highest values of available soil K were obtained at 30 DAT when rice plants were fertilized with 92 kg N fed^-1 combined with application of full dose of potassium before transplanting in 2007 and 2008 seasons, respectively. The highest values of nitrogen and potassium uptake were recorded at 30 days after transplanting (DAT) when rice plants were fertilized with 92kg N fed^-1combined with application of potassium full dose (50kg potassium sulphate fed^-1) before flooding at both seasons of study. Data showed also that maximum nitrogen uptake values were found when 92kg N fed^-1 was combined with application of potassium (half dose before transplanting +  spray with 2% K₂O (4kg potassium sulphate) at panicle initiation stage + spray with 2% K2O (4kg potassium sulphate) at flowering stages). The N uptake increased significantly at 50, 70 and 95 days after transplanting (DAT) in both seasons of study. Data showed also that application of nitrogen at the rate of 92 kg N fed^-1  in combined with application half dose of recommended amount potassium before transplanting + spray with 2% K₂O at panicle initiation and flowering stages gave the highest grain yield in 2007 and 2008 seasons, respectively.