A pot experiment was conducted at El-Mansoura laboratory of plant nutrition; Agric.Res.Center, during the tow seasons of 2008 and 2009  In order to evaluate the effects of Azotobacter inoculation with and without organic and mineral fertilization on yield and chemical composition of tomato plants. A factorial experiment in the form of complete randomize block design with three replicates has been used. NPK fertilizers were added at the 0,50,75 and 100% of the recommended dose for tomato crop once with inoculation by Azotobacter and the other without inoculation. All treatments were investigated in the presence and absence of organic manure (FYM) giving a total of 16 treatments. Results revealed that inoculation of tomato seedlings with Azotobacter sp. in combination with the rates of NPK fertilizers either with or without FYM significantly gave higher magnitudes of plant growth, yield and its component and chemical composition parameters than the uninoculated treatments. Raising NPK applied levels to 100% of the RD gave insignificant difference during both seasons. In addition, stimulation effect was happened due to using FYM combined with the same rates of NPK fertilization either in the presence or absence of Azotobacter inoculation but the rates of increases over the control treatment were more pronounced during both seasons. The intermediate levels of NPK; 50%+Azotobacter+FYM, seemed adequate and was associated with the highest mean values for the previously mentioned traits.