Effect of organic fertilizer as compost (20 m³ fed^-1), N-biofertilization (cyanobacteria Azospirillum, Azotobacter inoculations and mix of previous inoculations) and mineral nitrogen fertilization application rates (0, 30, 60 and 90 kg N fed^-1) on wheat grain and straw yields, N-uptake and nitrogen utilization efficiency (NU_tE) were studied for wheat crop (Triticum aestivum, L.), variety Sakha 93. Two field trials at El-Serw Agricultural Research Station, Dammietta governorate through winter season 2011/2012 and 2012/2013 were conducted. The results showed that wheat grain and straw yields and N-uptake in grain and straw increased with use of mineral fertilizer rates up to 90 kg N fed^-1, but N utilization efficiency for wheat crop was decreased. As well as these results showed that a mixture of bio-fertilizers, cyanobacteria, Azotobacer and Azospirillum inoculations, respectively gave the highest values of the previous parameters except the NU_tE, where the order of values was upward with the order of the previous inoculations. Also, the results showed that the use of organic fertilizer as a compost gave the highest values of wheat grain and straw yields and N-uptake in grain and straw and the lowest values of NU_tE. Organic fertilizer + a mixture of bio-fertilizers + 90 kg N fed^-1 gave the highest wheat grain and straw yields and N-uptake and gave the lowest N utilization efficiency. In addition, applying organic fertilizer + mix or BGA or Azotobacer inoculation could produce economic wheat grain and straw yields when it combined with 30 kg N fed^-1 and in the same time, this treatment gave high N utilization efficiency and thus saves in mineral fertilization, which may lose when applying the full recommended dose and conserves environment by reducing pollution hazards.