Effect of seed inoculation with the rhizobacteria Azospirillum brasilense AC1 and Micrococcus roseus SW1 in combination with P fertilization at levels of 0, 50 and 100% of the recommended dose on yield and nutrient contents of wheat plants grown on a sandy soil was studied in a greenhouse pot experiment. The results showed that grain, straw and biological yields of wheat were significantly improved with raising P- level. Also, the seed inoculation with the two bacterial strains caused significant increases in both grain and straw yields as compared to the uninoculated control. In addition, both yield parameters were significantly higher with the strain AC1 than with SW1.The highest grain and straw yields were obtained with 100% P dose plus A. brasilense at which the increments over the corresponding uninoculated control were 44.2 and 50.9%, respectively. The uptake of N, P, Fe and Mn by grains as well as the total uptake of N, Zn and Cu by plant were significantly increased at 50 and 100% P doses as compared to the control, but the differences between the two P fertilizer doses were not significant. The uptake of K by grains and straw and uptake of P, Fe, Mn and Zn by straw as well as the total uptake of P, K, Fe and Mn by plant were also significantly increased with increasing P fertilization level up to 100% dose. The uptake of all measured nutrients (N, P, K, Fe, Mn, Zn, Cu) by grains and straw were significantly increased by seed inoculation with the two tested strains relative to the uninoculated controls. The foregoing results revealed that the effectiveness of the tested strains individually for improving yield and nutrient uptake by wheat decreased with increasing the applied levels of P. The results indicated also that inoculation of wheat with the two mentioned rhizobacterial strains improved yield and nutrient contents in plant. The dosage of P mineral fertilizer currently applied for wheat in the Egyptian sandy soils might be reduced by combination of A. brasilense AC1 inoculation plus 50% of the recommended superphosphate dose. These results are recommended for field evaluation under different soils and environmental conditions before generalization of the two tested strains as biofertilizers.