In order to study the effects of soybean cultivars (Giza 111 ,Giza21 ,Giza22 ,Giza35  and  Crawford)  as well as to compare inoculation with non-inoculation of  PGPR (Plant Growth –Promoting Rhizobacteria ) as biofertilizers on seed yield ,proline content ,protein% , oil% and the uptake of macro-micronutrients in different cultivars of soybean, two experiments were conducted at  El-Quntra Shark Farm, East of Suzie Canal, Ismalia Governorate, The site lies in the north-waste in coast of Sinai, between 32^o- 35' and 32^o – 45' E and 31^o- 00' and 31^o -  25^o N, (Kaiser, 2009) , Egypt during the two successive summer seasons of 2012 and 2013. The experimental plots were divided into two main groups. The first group was without bio-fertilizer and treated only by one rate of 30kg N fed^-1 which applied as urea (46 % N). The second group was treated with bio-fertilizer combined with 20 kg N fed^-1.  The obtained results reported that seeds of Giza 35 and Crawford cultivars without using biofertilizer showed a significant increase in seed yield, weight of pods (Mg/fed) and 100-seed weight (g), concentration and uptake of macro and micronutrients. Also, the untreated seeds significantly affect on proline content, protein and oil percentage; while Crawford cultivar recorded the lowest value of proline and the highest values in protein and oil percentage. The inoculation of seeds for different cultivars of soybean gave the highest values of seed yield, weight of pods, protein and oil percentage and low proline content in comparison to untreated seeds. Also, the seeds treated with biofertilizer recorded a significant increase in the concentration and the uptake of macro and micronutrients.  The interaction between cultivars and inoculated seeds indicated that Giza 35 surpassed the other cultivars in seed yield, weight of pods, 100-seed weight and was significant in uptake of Fe, Mn and Zn in the first season, while, in the second season, Crawford cultivar   gave the highest values in seed yield, weight of pods and the uptake of Mn and Zn as well as gave significant increase in concentration of   Fe, Mn and Zn in both seasons. Proline content was significantly response to inoculation with biofertilizer in Giza35 and Crawford cultivars and gave the lower values than the other cultivars, while protein and oil % were significant increase in the same cultivars. The data reveal that according to the interaction, Giza 22 cultivar recorded highly significantly enhancement for P% only in both seasons and highest uptake of P and K for the second season. The analysis of soil after soybean harvested showed that soil pH and EC values were lowered in all the studied cultivars. Also, the available content of N, P and K were significantly increased, while, the available of Fe, Mn and Zn were non-significant in comparison with untreated seeds.       Therefore, the different cultivars of soybean inoculation with Rhizobium radiobacter (PGPR) not only decrease nitrogen fertilizer application, but also improves soybean yield and yield components. We concluded that Rhizobium radiobacter (PGPR) could be an eco-friendly alternative for reducing soil pollution caused by fertilizers usage and reduce the impact of soil salinity.