Two field experiments were conducted in Galbana village, North Sinai Governorate during two winter seasons 2011/2012 and 2012/ 2013, to evaluate the environmental effect of mineral nitrogen fertilizer rate, compost and bio-fertilizer on some soil properties and sugar beet productivity under newly reclaimed saline soil.Theseeds of sugar beet (Beta vulgaris) variety Loilwere inoculated with Rhizobium radiobacter strain (salt tolerant PGPR). Results indicated that the soil pH was not significant as affected by bio-fertilizer or compost alone or in combination with different rates of mineral nitrogen fertilizer. The soil salinity decreased with increasing rate of mineral nitrogen combined with bio-fertilizer or compost. The relative increases of soil available N content in soil due to mineral nitrogen fertilizer; compost and bio-fertilizer after sugar beet cultivation, followed the descending order: bio-fertilizer> compost > mineral N as compared with initial soil for  available N content. The relative increases in both P and K followed the descending order:  compost > bio-fertilizer > mineral N fertilizer as compared with soil initial contents of P and K. The available micronutrients ie Fe, Mn, and Zn in soil were not significantly affected by the different fertilization treatments in both the first and second seasons. Also, available Fe was not significantly affected with rate of the used fertilizers whereas the effects of different rates on the available contents of both Mn and Zn were significant in the second season, however in the first one such rates were of significant effect of Zn only. The interactions among bio-fertilizer, compost and mineral nitrogen rates were of significant effects on Mn content in soil in both seasons whereas such an effect was significant on Fe in both seasons and Mn in the first one. The highest mean values of fresh and dry root yield, sugar yield, total soluble solids (TSS), purity and sucrose were achieved due to treating soil with bio-fertilizer together with the mineral nitrogen fertilizer. The highest values of N (1.14 %) concentration in root was observed at soil treated with compost plus 100 kg mineral N fertilizer, while the maximum values of P and K concentration  (0.28 % for P and 1.29 % for  K ) was observed as affected by bio-fertilizer plus 100 kg mineral N, respectively. On the other hand, the effect of all treatments tested on Fe, Mn and Zn concentration in root of sugar beet was non significant. As a conclusion, bio-fertilizer and compost application in sugar beet could increase characteristics of sugar beet root and reduced consumption of mineral nitrogen fertilizer and successfully reduced the hazard effect of soil salinity condition.