The present study was carried out on EL-Serw drain located between Dakahlia Governorate and Damietta Governorate to evaluate the effect of drainage water quality on chemical composition of soil and plants. EL-Serw drain passes through many villages dotted along it receiving their agricultural drainage water, domestic waste waters and industrial effluents. Water samples were seasonally collected from 6 sites along this drain (the beginning of the drain (0), 5, 10, 15, 20 and 25 km.) during June 2012 to March 2014. The main obtained results are presented as follows: Electrical conductivity (EC) values increased slightly with northward direction. Also, Sodium adsorption Ratio (SAR) took the same trend, the quality of studied drainage water belong to  C3 S1 and C4 S2 classes and could be reused  in irrigation purpose under special management. Boron (B) concentration, at most of locations  have low concentration (B1) < 3 mg L^-1 which less than the critical limit indicating (slight to moderate for restriction on use). Nitrate –N concentration was ranged from 5-30 ppm in the EL-Serw drain in two summer season 2012 at distance 0 to5 Km. and 2014 at distance 0 to 10 Km. whereas, the  other sites had higher water than the critical limits. Micronutrients and heavy metal ions concentrations (Fe⁺², Mn²⁺, Zn²⁺ and Pb²⁺) were less than permissible levelsat all selected water samples. Soil salinity and SAR values of soil increased as a result of using drainage water and the increasing of soil pH is related to salt content in irrigation water. Using drainage water for irrigation, caused an increase in soil content of available micronutrients (Fe, Mn and Zn) whereas, heavy metals (Pb) was less than the permissible limit (500 mg Kg^-1). Concentrations of heavy metals and micronutrients (Fe, Mn, Zn and Pb) in straw and grains of wheat and rice grown in the most locations were under the permissible limits except Pb and Fe concentration exceeds the critical levels in all locations.The concentration of heavy metals and micronutrients in straw of wheat and rice crops were higher than in the grain in all locations. Zn and Mn concentrations were less than critical limits in all locations in shoot of clover plants and Pb and Fe concentrations were higher than critical limit in all locations.  Finally, the rice crop slightly affected by water quality than wheat and finally clover.