A pot experiment was conducted in the greenhouse of Soils and Water Research Department, Atomic Energy Authority, Egypt, in the winter season of 2011/2012. Four irrigation water differed in EC were used in the experiment: 0.25 dS m^-1 (Fresh water as a control), and three saline irrigation water 2, 4 and 6 dSm^-1. Basic supplement of P and K fertilizers were applied to each pot, at rate of 200 mg kg^-1 soil as super phosphate and 50mg kg^-1 soil as potassium sulfate, respectively. Ammonium sulfate was added at rate of 120 mg N kg^-1. Proline was added at three concentrations, zero, 15 and 30 mg l^-1 and sprayed in the early morning. Data showed that inoculation with Azotobacter alone led to a significant increase in dry weight of wheat plants and weight of spike compared to uninoculation, regardless of the addition of saline water. Data obtained also indicated that the increasing concentration of spraying proline acid from zero to 30 ppm in most cases lead to increase growth and dry weight of wheat crop as well as the weight of spikes. This holds true at the different levels of saline irrigation water or even added to tap water (Control). The concentration of sprayed proline (30 ppm) in most cases gave the highest value of dry matter yield of both plant and spikes. Nitrogen uptake by plants tended to decrease with increasing irrigation water salinity levels. Severe decrease was noticed with salinity level of 6 dS m^-1. Gradual decrease in nitrogen uptake by plants was detected with 2 and 4 dS m^-1 salinity levels as compared to control treatment. The adverse effect of salinity levels of irrigation water was slow down by proline additions. Enhancement of nitrogen uptake was induced by addition of proline at 30 ppm concentration, followed by those of 15 ppm concentration.