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48310

Response of Rhizobium-Faba Bean Symbiosis System to Rhizobacterial Inoculation and Arbuscular Mycorrhizal Fungi under Graded Levels of Natural Rock Phosphate in Sandy Soil

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Last updated: 22 Jan 2023

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Abstract

Two field trials were conducted during two winter growing seasons of 2016/2017 and 2017/2018, using drip irrigation system. The target of this study was to evaluate the response of Rhizobium-faba bean symbiosis system to rhizobacterial inoculation (Serratia  marcescens and Bacillus megaterium) and AM-fungi under graded levels of natural rock phosphate (15,30 and 45 kg P2O5/fed).The experimental design was arranged in a completely randomized block design with four replicates. The number and dry weight of nodules, N2-ase activity, plant height, dry weight of shoot and their contents of N, P and K ,the number of pods plant-1 and the number of seeds pod-1as well as 100-seed weight, the biological yield, seed yield and seed crude protein percentage were evaluated. The study was also concerned with the impacts of such microbial inocula and rock phosphate on dehydrogenase and phosphatase activities in soil rhizosphere. The obtained results elicited that the uninoculated plants were poorly nodulated. While, inoculating faba bean seeds with efficient Rhizobium exerted great improvement in nodulation status and N2-ase activity. The microbiological properties of rhizosphere soil, which expressed by dehydrogenase enzyme activity and the activity of phosphatases displayed higher response to applied biofertilizers. In fact, the splendid effect was observed with the use of inoculation approach (mixture of Rhizobium, Serratia, Bacillus and AM-fungi), which caused promotive impression in nodulation, the activity of rhizosphere soil enzymes, plant growth aspects and all faba bean yield charactersin relative to other combinations or plants inoculated with Rhizobium only. Irrespective of inoculation, there is an increase in nodulation status, all vegetative growth characters, the activity of rhizosphere soil enzymes and faba bean yield with increasing natural rock-phosphate rate from 60 to 180 kg/fed(15 to 45 kg P2O5/fed).Hence, data confirmed the superiority of treatment comprising (Rhizobium conjugated mixture of PGPR's and AM-fungi)in combination with 30 or 45 kg P2O5/fed, which surpassed the other tested combinations and caused a significant augmentation in all studied faba bean parameters. While, inoculation with Rhizobium combined with PGPRs or AM-fungi and fertilized with 45 kg P2O5/fed came in the second rank. Obtained findings displayed a considerable evidence that inoculation of faba bean seeds with Rhizobium combined with a mixture of rhizobacteria may revealing a good practice for improving faba bean growth and yield characters and leading to healthier food, particularly when this practice supported by effective AM-fungi and natural rock phosphate (30 kg P2O5/fed).

DOI

10.21608/jssae.2019.48310

Keywords

Faba bean (Vicia faba L.), Rhizobium leguminosarum, Serratia marcescens, Bacillus megaterium, Arbuscular mycorrhizal fungi (AM-fungi), Rock-phosphate and Sandy soil

Authors

First Name

Sh.

Last Name

Abdel-Gawad

MiddleName

A.

Affiliation

Agric. Microbiol. Dept., Soils, Water and Environ. Res. Inst., ARC, Giza, Egypt.

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First Name

M.

Last Name

Aboel -Enin

MiddleName

M. M.

Affiliation

Agronomy Dept. Fac. Agric. Al-Azher Universty, Cairo, Egypt.

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Volume

10

Article Issue

6

Related Issue

7513

Issue Date

2019-06-01

Receive Date

2019-09-17

Publish Date

2019-06-01

Page Start

363

Page End

375

Print ISSN

2090-3685

Online ISSN

2090-3766

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https://jssae.journals.ekb.eg/article_48310.html

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https://jssae.journals.ekb.eg/service?article_code=48310

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5

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Original Article

Type Code

889

Publication Type

Journal

Publication Title

Journal of Soil Sciences and Agricultural Engineering

Publication Link

https://jssae.journals.ekb.eg/

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Article

Created At

22 Jan 2023