In this study the effect of salinity stress on growth and macronutrient uptake of some barley landraces ((Hordeum vulgare L.) grown in sandy loam soil were investigated. The main purpose of this study was carried out to evaluate three Egyptian barley landraces i.e. (L2, L6 and L10) for salinity tolerance. The plants were irrigated by using saline water with different concentration (0, 50.100 and 150 mM NaCl) for 60 days. A pot experiment was conducted to achieve this study. Results indicated that increased salinity caused a significant reduction in percentage of fresh and dry weights of barley plants. Soil pH and EC values were increased as a salinity level of irrigation water increased. Soluble Na⁺ concentration were increased with increasing NaCl levels, whereas Ca, Mg and K in soil were decrease as a result of increasing the concentration of NaCl in irrigation water.  That is true for all landraces. In addition, the values of N, P and K content in barley landraces were decreased with increasing salinity levels. Also the N, P and K uptake were significant decreased by increasing salinity levels.  Generally, the results clearly showed that landrace L2 had the highest salt tolerance compared with landraces L6 and L10.