Two field experiments were conducted to study the effect of bio and organic fertilizer as a partial alternative to mineral-nitrogen fertilizer, on growth, yield and quality of maize plants (c.v. tri- hybrid 324). The study was carried out during the two successive seasons of 2013 and 2014 at El- Gemmeiza Research Station(30º 47׳ 22.5״ N- 31° 07׳ 34״ E, elev. 10 m), Agricultural Research Center, El-Gharbia Governorate,Egypt. Significant positive influences of 50% of the recommended N rate (120kg N/fed.) +25%of the recommended N rate as organic N +Bio  treatment were observed on growth traits after75 days from planting, leaf contents of nitrogen, phosphorus, potassium and yield quality when compared to the other combined treatments. The same treatment, gave the highest grain yield (4450 Kg/fed) which was 9.88 % higher than the ₍100% mineral N) control (4050Kg/fed) and 51.4% more than the lowest yield of 50% M.N.F. (2940 Kg/fed). Also, results indicated that the treatments comprising 50or 75% mineral N + 4.2 or 2.1 ton/fed. organic N +Bio and/or humic acid significantly increased plant height, weight of 100 grain  , grains, straw  and biological yields  than control. All quality parameters i.e. protein, oil %, total carbohydrate and starch contents under 50% mineral N + 4.2 ton/fed. organic N +Bio and/or humic acid treatments recorded the highest and significant values comparing to the control. Hence, it can be concluded that organic and biofertilizers could be used to minimizing the amount of mineral N fertilizer fot different crops avoiding soil or water pollution.