The impact of irrigation with wastewater supplied from drain No. 7, Kafr El-Shiekh, Egypt on wheat plant, was investigated. Growth properties, photosynthetic pigment content and the antioxidant system of wheat plant were estimated. Furthermore, heavy metals contents in the collected samples of soil, water from different sites and heavy metals accumulation in the studied plants. For this purpose, two groups of wheat plants were considered. The first group obtained from fields which irrigated using fresh water, while the other group was irrigated using water of drain No.7 from the selected five sites of drain 7. The obtained results revealed that drainage  water contained significant amounts of heavy metals (Cd, Ni and Cu) warranted a pollution problem as their amounts exceed the maximum recommended concentrations according to FAO guidelines for trace metals in irrigation water. The wheat plants accumulate significant amounts of heavy metals in their shoots, straw and grains and showed a significant decrease in, fresh weight and dry weight of shoots and roots, accompanied by a marked reduction in photosynthetic pigment content. Significant increase in the activities of antioxidant enzymes and in total  carbohydrate content in response to irrigation with wastewater. Cadmium was higher than critical levels at all sites in water. Cupper, Cd and Pb concentration at all sites were lower than critical levels in soil. Cupper,Cd and Pb concentration (ppm) in grains and straw of different sites were increased gradually from site 1 up to site 5. Lead values were the highest and Cd values were the lowest in grains and straw, but Pb concentrations at straw were higher than in grains. Cupper, Cd and Pb concentrations were lower than critical levels in grains. Cupper and Cd concentration were lower than critical levels, but Pb concentration was higher than critical levels in straw.