Two field experiments were conducted at the Experimental farm of Sakha Agric. Res. Station during 2012/2013 and 2013/2014 growing seasons to evaluate the potential effect of addition of humic substances and cyanobacteria (mixture strain of Anabeana oryzae and Nostoc muscorum), on wheat (Giza 168) yield and its components. The experiment layout was split plot design. The main plots were assigned by application methods where three application treatments (soaking, foliar and soaking with foliar) were used. The subplots were assigned by four treatments of cyanobacteria, H.S and without. Results indicated that inoculation with cyanobacteria and H.S significantly increased grain and straw yields. The yield and yield components, chlorophyll content, N uptake and protein % in grain were increased over their values with the untreated plants in both growing seasons. The obtained results indicated also that treatment of (soaking + foliar) with cyanobacteria + H.S significantly increased the yield compared with other application method, the highest values of grain yield 23.79 and 23.43 ardab/fed., straw 4.82 and 4.84 ton/fed., protein 11.73 and 11.6%, total chlorophyll 43.65 and 43.48 spad unit and plant height 89 and 87 cm with (S.+F.) treatment.