A field experiment was carried out during the years 2013, 2014 and 2015 on Eureka lemon at El-Nubaria region, El-Beheira governorate, to study the effect of farmyard manure and biofertilizers with NPK dose on soil fertility, growth, yield, fruit quality and leaf nutrient content of Eureka lemon trees. Eleven treatments were arranged in a randomized complete block design with three replicates. Results revealed significant differences amongst various growth attributes, fruit yield, fruit quality, leaf mineral content and soil nutrients availability due to farmyard manure and biofertilizers application. Also, farmyard manure and biofertilizers could compensate for the 50% reduction of chemical fertilizers, and increasing yield compared to chemical fertilizers with significant differences. Therefore, treatment of 50% NPK + 55 kg farmyard manure + biofertilizers is recommended for growers. This treatment gave the best growth, yield and fruit quality of Eureka lemon trees. Moreover, this treatment improved the nutritional status of the trees, through the beneficial effects of organic and biofertilizers which enhanced the availability of most nutrients in the soil.