A field experiment was conducted during two successive winter growing seasons of (2012/ 2013 and 2013/ 2014) at the experimental farm of Mallawi Agricultural Research Station, Mallawi center, EL- Minia Governorate, Egypt. The objective of this research was to study the effect of applying different levels of inorganic nitrogen (0, 50, 75 and 100% N fed^-1 from RD 75kg N fed^-1) and organic fertilization (0, 5 and 10 ton fed^-1) and inoculation with Azospirillum brasilenes as well as their interactions on number of bacteria yield, yield quality, and nitrogen content and uptake by wheat plants cultivar (Beni suef one) grown in silty clay loam soil. The results showed that increasing the level of mineral nitrogen fertilization from 0, 50, 75 and 100% N fed^-1 from RD of nitrogen (75kg N fed^-1) resulted in a significant increase in all studied parameters (1000 grain weight, grain yield, straw yield, protein percentage in grains, N content in grain and straw, nitrogen uptake by grain, nitrogen uptake by straw and total nitrogen uptake by grain and straw except number of Azospirillum brasilenes per gram soil was not significant. All studied parameters were significantly increased due to increasing the level of organic fertilizer from 0 to 5 ton fed^-1 or 10 ton fed^-1 except nitrogen uptake by the grains in the first season was not significantl. Inoculation of wheat plants with Azospirillum brasilenes resulted in a significant increase in number of Azospirillum brasilenes per gram soil, 1000 grain weight, N content in grain in the second season, N content in the straw, nitrogen uptake by grain in the first season,   total nitrogen uptake in the second season and protein percentage in the second season only, While, results was not significant increase in grain and straw yields, N content in grain in the first season, nitrogen uptake by grain in the second season, nitrogen uptake by straw, total nitrogen uptake in the first season and protein percentage in grain in the first season. The highest values of studied parameters were obtained when wheat plants were fertilized with mineral nitrogen fertilizer at the rate of 75% from RD of nitrogen (75kg N fed^-1) combined with 10 ton fed^-1 organic fertilizer and inoculated with Azospirillum brasilenes. The effect of the interactions between the three studied factors on most the studied parameters was significant increase except the number of Azospirillum brasilenes per gram soil, grain yield in second season, straw yield in the second season, nitrogen uptake by grain in the second season and nitrogen uptake by straw in the second season and total nitrogen uptake in the second season was not significant. The results indicated that application of mineral nitrogen fertilizer at the rete 56.25 kg N fed^-1 (75% from RD 75 kg N fed^-1) combined with organic fertilizer at rate 10 ton fed^-1 to the silty clay loam soil cultivated with wheat plant and inoculation with Azospirillum brasilenes improved quantity and quality of wheat yields.