Field experiment was conducted at the Agricultural Faculty Experiment Farm of New Valley Branch, AssiutUniversity at New Valley Governorate, Egypt, during 2014/2015 and 2015/2016 seasons to study the effect of chicken manure fertilizer rates (0, 3 and 6 ton.fed^-1) on yield and its components of some barley cultivars. The experimental design was randomized complete block design (RCBD) in split plot arrangement. The results indicated that Giza 123 and Giza 134 were superior the rest cultivars in almost studied traits. Increasing rates of chicken fertilizer from 0 to 3 or 6 ton.fed^-1 resulted in an increase in all studied traits under this study except harvest index which was decreased. The interaction affects significantly biological yield and spike length in both seasons, while for grain yield was in the first season, and straw yield in the second season. The application of chicken manure by the rate of 3 or 6 ton.fed^-1 reducing the mean values of soil pH and calcium carbonate (CaCO₃) content after the first and second seasons. On the other hand, the electric conductivity (EC) and organic matter (OM) levels were significant increased with increasing the level of chicken manure. The high level of chicken manure (6 ton.fed^-1) gave the highest mean values of N, P, and K after the first season than the second season.