Field experiment was carried out at EL-Ismailia Governorate in the winter season of 2013-2014 to evaluate the role of Azolla pinnata, Anabaena Azolla, Pleurotus columbinus and Azotobacter sp in the presence of urea (46.5% N) as source of nitrogen fertilizer on growth and yield of wheat. It was found that total count bacteria, fungi, azotobacter and algae at different treatments were higher than those of other treatments especially with treatment (T16) of Mix only which gave the highest values of total bacteria count 4000 x 10³ cfu g^-1 dry soil, followed by (T₁₇) which recorded 3830 x 10³ cfu g^-1 dry soil.Also for different microbes such as fungal, algae and N₂-fixing bacteria counts it was noticed that treatment T17 (Mix of biofertilizer+ 75% of recommended dose of nitrogen) gave the more optimum results for different types of microbes count. The results showed that (T18) and (T17) recorded the highest values for IAA production which were (69.92 ad 68.7 mg ml^-1g^-1 dry soil). Where T16 and T17 gave mostly higher N₂-ase activity (32.36 and 21.97µ C₂H₄ g^-1dry wt. hr¹) at 120 days of incubation compared to other treatments.Also straw and grain yield was significantly increased with mixture +75% of recommended dose of nitrogen fertilizer (T17) (1365.04 g/m²) straw and (999.83 g/m²) grains. Also it was noticed that treatments of mixture of biofertilizer have a pivotal role in increasing N, P, and K uptake in straw with treatments T16, T16 and T17 respectively. Whereas with grain yield the treatments T17, T16 and T16 gave the highest values of wheat grain, respectively.