A pot experiment was conducted outdoor at the Experimental Farm, Faculty of Agriculture, Mansoura University during the summer seasons of 2015 and 2016.to evaluate the positive interaction effects among different types of organic fertilization forms and levels alongside with bio fertilizer applications on quantitative and qualitative yield characteristics of cucumber grown under an organic farming conditions.. Thirty treatments were arranged in a split-split plot design with three replicates, which were the simple combination of three types of organic amendments (compost, FYM, and biochar), two rates of soil application (5 and 10 ton fed^-1) and five types of bio fertilizer application forms i.e. (1) microbien + phosphorien, (2) microbien + phosphorien+ effective microorganisms (EM), (3) EM, (4) poultry manure extract, and (5) control (without biofertilizetion). Compost proved its effectiveness in improving yield characteristics, nutrients content and quality indices as compared with other organic amendments. The application level of 10 ton fed^-1 was the optimum rate for providing sufficient needs of plant during its whole growth stage. The combined biofertilization treatment (microbien + phosphosien+ EM) was the most effective treatment for improving quantitative and qualitative yield characteristics. The obtained results concluded that the integrated treatment of compost application at 10 ton fed^-1 alongside with microbien + phosphosien+ EM biofertilization is recommended to produce the highest productivity and quality indices of cucumber yield grown on a sandy soil condition.