A field experiment was carried out on sandy soil of Agric. Res. Station, at IsmailiaGovernorate, Egypt lat. 30° 35' 41.9"N, long. 32° 16' 45.83" E. during two successive winter seasons of 2013/2014 and 2014/2015 to studied the response of wheat plant (Sakha 93) to nitrogen fertilization in two forms i.e., ammonium-N and nitrate-N in different ratios at the rate of (100 Kg N fed^-1) as ammonium nitrate (AN) as a source of NH₄-N and NO₃-N and anhydrous ammonia (AA) as a source of (NH₄-N).The obtained results showed that the most effective balance which gave the highest significant increases in all yield parameters was (12.5 Kg NO₃-N+ 87.5Kg  NH₄-N ) comparing with Applying AN (50 Kg NO₃-N+ 50 Kg NH₄-N) alone and the average of improvements were 11.6%  , 52.3% , 42.2  and 72.9 % for 1000 grain weight , grain yield , harvest index  and yield efficiency, respectively.In addition, Applying (100 kg N/fed as AA) resulted in slightly increase in straw and biological yields compared with (12.5 Kg NO₃-N + 87.5 Kg  NH₄-N ) but it was not significant and recorded increase about 35.5% and 39.8% ,respectively. The results indicated that application of  AN in combination with AA (12.5 Kg NO₃-N / 87.5 Kg  NH₄-N) exhibited the best treatment in enhancing the uptake of N,P, K and protein yield in wheat grains as it achieved 82.3% ,104.8%,84.9% and 82.3% increase ,respectively. Furthermore, increasing rate of NH₄-N as anhydrous ammonia (100 kg N fed-1) in the absence of NO₃-N gave the highest N and P uptake in straw by 99.8% and 72.6% increase for both elements. While, the highest value of K uptake in straw had increased by 53.7% with combined treatments of 75Kg AN+90 Kg AA /fed (12.5 Kg NO₃-N + 87.5 Kg NH₄-N) and significantly surpassed the single AA alone.However, usage of ammonium nitrate fertilizer in combination with anhydrous ammonia fertilizer had noticeable effect for lowering the quantity of ammonium nitrate fertilizer and improvement the yield quantity and quality of wheat plant.