The   present   work   aims   to   study   the   effect   of   anaerobic   fermentation temperature  on  the  production  of  ethanol  fuel  for  three  different  types  of  sugar compositions in dates that commonly produced in Saudi Arabia. The fermentation temperature is considered as the most important factors affecting the production of ethanol. The obtained data referred that the ethanol yields from Sukkari dates were 107.6 cm³ L^–1  juice, 123.3 cm³ L^–1   juice,  and  96.6  cm³ L^–1 juice,  during anaerobic fermentation temperatures  of  30°C,  35°C,  and  40°C,  respectively. Whilst,  the ethanol yields  from Khlass  dates  were  111.0  cm³ L^–1 juice,  122.0    cm³ L^–1 juice, and 128.0 cm³ L^–1 juice during the same fermentation temperatures. Ultimately, the Berhi dates produced 121.0 cm³ L^–1 juice, 136.0 cm³ L^–1 juice, and 136.0 cm³ L^–1 juice, under the same fermentation temperatures.  The obtained results showed that the production of ethanol would directly proportional to the ratio of glucose and fructose composition in dates.