A factorial randomized complete block field experiment was carried out on lettuce (Lactuca sativa var. longifolia cv. Balady), grown on a heavy clay torrifluvent soil during two successive seasons (2016/2017 and 2017/2018) at the farm of the Faculty of Agriculture, Moshtohor, Benha University to study foliar application with nano-urea and biofertilization using Vesicular Arbusclar mycorrhiza (VAM).  Factor 1 included 2 treatments: none (B₀) and VAM (B₁); Factor 2 included 5 treatments: no urea spray (spray with water) (N₀), spray with ordinary urea at 5000 mg N L^-1 (N₁), spray with nano-urea at 2500 mg N L^-1 (N₂), 3750 mg N L^-1 (N₃) and 5000 mg N L^-1(N₄). Spray was in 3 times 30, 40 and 50 days after transplant at a rate of 1200 L ha^-1each time. All growth parameters of plant height, number of leaves plant^-1, fresh and plant dry weight increased by N or VAM singly or combined. Highest positive response occurred in plant height as well as weight and given by N₃B₁. All nano-urea treatments surpassed the ordinary one, particularly the middle nano rate. The highest nano rate seemed excessive since N₃ did not continue to score further increase. Contents on N, P and K increased by nano-urea. NO₃-N increased progressively with increased application of urea, and the increase progressed with increased rate of nano-urea. The nano application proved practical and more efficient since a concentration of as low as 2500 mg N L^-1 proved superior to that of 5000 and mg N L^-1 ordinary urea N.