Nanotechnology is a novel discovery being explored in almost all the fields and is benefitted too; it may provide keener solutions for the current problems in the field of agriculture. Nanotechnology and bio fertilization are represented the most important tools in modern agriculture and anticipated to become a driving economic force in the near future. A field experiment was carried out at Baloza Research Station of Desert Research Center, North Sinai (located at 31° 3′ 0″ N, 32° 36′ 0″ E), to study the effects of application for nitrogen fertilizer (urea), nano urea and biofertilization (Azotobacter chroococcum), on the chemical composition and productivity of sage plant (Salvia officnalis L.). Two mineral fertilizers, nano urea, urea and biofertilizer ( A. chroococcum)  were applied  to the soil.  Split split plot design with three replications per each treatment was used, during 2017. Results showed that yield components of sage plants increased with increasing of nano urea and urea application rates during both cuts. The mineral treatments with bio N fertilizer gave the highest yield values. The most effective treatment (Nano 500ppm with bio N fertilizer) gave the highest significant values of yield components  amounted to 64.4, 2.77, 11249 and 4395 for   plant height (cm), oil (%), herb fresh and dry weight (kg/fed), respectively in first cut. In second cut yield components were higher than first cut. Nutrients contents and then uptake showed almost trend. The N-efficiency parameters; the highest values of nitrogen use efficiency (NUE) and nitrogen uptake efficiency (UPE) were obtained by the application of nano urea. The values of nitrogen utilization efficiency (UTE) and NUE decreased with increasing fertilizer application while that of UPE increased with increasing nitrogen.  The superior treatment Nano 500 with bio N fertilizer highly increased the available nutrients in the studied soil. The same effect was observed on the values of dehydrogenase activity and Azotobacter densities in rhizosphere of Salvia officnalis.