A field experiment was carried out at the Experimental Farm, Faculty of Agricultural, Mansoura University, Dakahlia Governorate, Egypt in 2015/2016 season to estimate response of Jews mallow plant (Corchorus olitorius L.) cv. Balady to nitrogen (N) fertilizer and foliar spraying with Fe plus Mo. Nitrogen was applied in five rates (0, 50, 100, 125 and 150 %) as NH₄NO₃ (33.5% N)  from recommended dose (RD) in the presence and absence of iron (Fe) plus molybdenum (Mo) as a foliar way, at the rates of 250 and 75 mg L^-1 Fe and Mo, respectively on vegetative growth parameters, chemical composition and quality parameters   for Jews mallow  plant.  A completely randomized block design was used, with 10 treatments with three replicates. According to results, vegetative growth parameters, chlorophyll a, b and total, vitamin C, total carbohydrates and concentration of P, K, Fe and Mo in Jews mallow leaves plant increased significantly with increasing nitrogen fertilization rate up to 100% from recommended dose (RD) at marketing stage. With increase in N level up to 150% RD, fresh weight, concentration of N, protein percentage, NO₃-N and NO₂-N contents in the leaves of Jews mallow plant were significantly increased. On the different of this tendency; nitrate reductase enzyme activity (NRA), vitamin C and total carbohydrates were significantly decreased with increasing nitrogen fertilizer rates from 100 to 150 % RD in the leaves of Jews mallow plant. Also, results showed that foliar spraying with iron (Fe) plus molybdenum (Mo) in 50, 100, 125 and 150 % N from recommended dose significantly improved vegetative growth parameters, quality parameters and chemical composition, in Jews mallow leaves. Analysis of NO₃-N and NO₂-N contents in leaves revealed that NO₃-N and NO₂-N concentrations decreases while nitrate reductase enzyme activity (NRA) was more pronounced increases in the presence of foliar spraying with iron (Fe) plus molybdenum (Mo) with all levels of N fertilization. Moreover, it is concluded that iron plus molybdenum at the rate of 250 and 75 mg L^-1 under 100% N from recommended dose increases vegetative growth parameters, quality parameters and chemical composition (except for NO₃-N and NO₂-N contents) in Jews mallow leaves. Thus protecting human health from the risk of accumulation of nitrates.