This experiment was conducted at the Agriculture Experiments and Research Center, Minia University during the two successive seasons of 2015/2016 and 2016/2017 to study the response of Moringa oleifera plants to mineral, organic and bio-fertilizers mixtures. Moringa seedlings were grown on soil enriched with 0, 5,10 and 15 ton/fed of compost as a main split plot, while the sub-plots included control, full dose of NPK,75%NPK+a mixture of bio-fertilizers "effective microorganisms (EM) + phosphorein", and this mixture of bio-fertilizers.The results showed that organic fertilizer, bio-fertilizers and NPK significantly improved the growth and seed yield of the Moringa plants in both seasons. The highest Moringa seed yield was for plant fertilized with bio-fertilization + 75% NPK and 15 ton/fed compost, whereas, the control plants had the lowest seed yield during both seasons. In all cases, bio-fertilization + 75% NPK was more effective in increasing all investigated growth and yield parameters than was 100% NPK or bio-fertilization under the same compost treatments. This trend was coincided with the plant content of the photosynthetic pigments and NPK content. From the results of this study, moringa plants grown under these experimental conditions achieved the highest growth and yield parameters following fertilization with bio-fertilization + 75% NPK at 15 ton/fed of compost.