The current study aimed to investigate the influence of using different sources of irrigation (well "ground water" and agri-aquaculture) with some seed treatments techniques on amount of water applied, water use efficiency, yield and economic analysis of sugar beet crop. An experimental was design on split plot with 6 treatments and three replicates, where three seed treatments techniques i.e. control, soaking (24 h) and soaking (72 h). Drip irrigation was used and crops were planted in rows. Data indicated that the amounts of water applied were 2653, 2565 and 2489 m3/fed. under control (C), soaking 24 h (S1) and soaking 72 h (S2). and amounts of water saving under different seed treatments (S1, and S2) compared with control treatment (C) were 88 and 264 m3/fed., respectively. These results demonstrated that values of both root and sugar water use efficiencies were 7.54 and 1.29 kg/m3 under well (W) treatment, while were 8.24 and 1.44 kg/m3 under agri-aquaculture (A) treatment. The highest value of germination ratio was 94.06% under S2 treatment, while the lowest value was 93.86% under C treatment. The maximum root yield among seed treatment techniques was recorded with S2 treatment (20.18 ton/fed) followed significantly by soaking seeds 24 h (19.19 ton/fed) and followed by control treatment (19.76 ton/fed). The highest net return and the maximum value of water productivity were 8427 LE/fed and 3.53 LE/m3 with used agri-aquaculture and soaking seed 72h, while the lowest value was 6144 LE/fed. and 2.32 LE/m3 with well water and without treated seed.