The effects of ascorbic acid on development and some biochemical analysis of Pectinophora gossypiella (Saund.) were investigated after feedingnewly hatched larvae with different concentrations of three ascorbic acid analogues i.e., L-ascorbic acid, D- isoascorbic and D- glucoascorbic acid. The added analogue and the used concentration significantly affected larval growth. Statistically, L-ascorbic acid seemed to be the best analogue, as the whole mean of survived larvae averaged 63.737% comparing with 54.17 and 32.9% for the D-isoascorbic and D-glucoascorbic acid analogues, respectively. All survived larvae succeeded to develop into pupae after feeding with any of the three concentrations of L-ascorbic acid, while, all larvae died or failed to develop into pupae when fed with 2.32 mg of D- isoascorbic or D- glucoascorbic acid analogue / 100g diet, respectively. The significant low percentages of survived larvae or produced pupae are a reflection to the reduction in the values of total protein, tyrosine and chitin in larval body. Statistical analysis of data yielded a significant correlation between the percentages of larvae which succeeded to develop into pupae and the quantity of their total protein (r = 0.8043**), tyrosine (r = 0.82277**) and chitin (r = 0.69874*) in larvae.