Twelve 9-frame colonies 1st hybrid (Carniolan x local Egyptian race) were chosen to study the effect of different concentrations (0.5, 1.0 and 1.5%) of pepperment oil. The reduction in Varroa population living on worker bees increased significantly by increasing concentration. The third application was more effective on infested brood cells than the first and second application. The effect of pepperment oil gradually reduced numbers of infested brood cells and was more significant after two applications. Mite mortality was significantly related with repetition of treatments and concentration used.