In Egypt, tomato crop is the second most important vegetable crop and Tuta absoluta comes among the most serious lepidopterous insects that attaching the tomato crop. Due to the dangers of chemical pesticides, nano-emulsions have recently been in pest control programs. The present study looked into the larvicidal activity of the three plant oil extracts and their nano-emulsions against the second instar larvae of T. absoluta. Spraying method was used for application on the leaves infested with T. absoluta larvae. Results showed that nano-emulsions of essential oils were more effective than traditional essential oils against larvae. The highest mortality proportion caused by the high concentrations of Nano emulsion of essential oils (250 ppm) and essential oils (10000 ppm) under laboratory conditions. But the highest reduction percentage in larvae population was caused by essential oil nano-emulsions under field conditions. As a conclusion, nanoparticles of essential oil have to be used as bio-pesticides in IPM programs of T. absoluta.