The present study was carried out at the apiary of Sakha Agricultural Research Station,  Kafr El-Sheikh Governorate during 2000 season to evaluate the effectiveness of four materials; formic  acid, oxalic acid, malathion powder and mitac 20% on varroatosis. The results indicated that all treatments were effective against the mite, but had little or no effects on the bees. Mitac 20% (acaricide) was the most effective compound on mites after a period of 8 days from treatment (620.6 mites), followed by Malathion (611.2), formic acid (212.6), while oxalic acid had the least effect (159.4 mites). The number of the associated mites was found to be  higher with drones than those with workers (510 and 329 mites/100 bees, respectively).