In the present investigation, some effective factors on natural mating successes of honeybee virgin queens were studied, such as: type of receiver colony race, introducing methods of virgin queen, presence of brood and their stages in receiver colonies for acceptance and mating of honeybee virgin queens. Results showed that carniolan bee colonies accepted the highest percentage (96.67%) of the introduces virgin queens, followed by both the first carniolan hybrid colonies and Italian colonies (93.33%), the lowest significant percentage of the accepted queens was recorded for the Egyptian virgin queens (70.00%). Concerning pre-mating periods of the introduced virgin queens, results revealed that there were no significant differences among periods of pre-mating required by the introduced virgin queens received by different bee colonies. Results of mating ability of the introduced virgin queens, Italian bees encouraged the highest percentage (78.88%). However queens introduced cambian and Egyptian bees colonies mated by the lowest percentage 65.92, 5416% respectively. Recording postmating periods of the tested queens showed that queens introduced to carniolan bees recorded a shortest penod (2.78 days), while the longest penod (3.37 days) was recorded to the first Italian hybrid. Results revealed that the highest percentage of acceptance (96.67%) was noticed with using half comb cage, while applying direct introducing method resulted in a lowest percentage (36.67%) of acceptance of the introduced queens. Through two years revealed that introducing virgin queens to the colonies have mixed brood resulted in acceptance of the introduced virgin queens was the highest rate (86.67 and 90.00%, respectively). On the contrary introducing virgin queen to colonies having only sealed brood resulted in the lowest acceptance percentage (66.67 and 63.33). Pre-mating period of the introduced virgin queens showed that the shortest period with introducing to the colonies of the colonies of the mixed brood , it was 3.06 days while with queen introduced to colonies having no brood 3.73 days. Mating success percentage should that although queens introduced to the colonies having mixed brood mated by the highest percentage (84.72%). Also1 in this colonies (mixed brood) helped in shorting the post mating periods of the introduced queens. The highest acceptance percentages through two years study (96.67 and 93.33% respectIvely) were recorded with introducing virgin queens to colonies 3 combs. However introducing virgin queens to colonies of 10 combs resulted ¡n decreasing acceptance level of these queens (53.33 and 56.76% for two years study). Concerning pre-mating periods of introduced queen no significant difference were found among different treatment. Recording mating success of the introduced queens, showed that queens introduced to colonies of 5 combs mated by the highest rate 78.88%, while the lowest level mating success (62.22%) was associated with those queens introduced to colonies of 10 combs.