The present field study was carried out at lsmailia Governorate during the two successive cotton growing seasons of 2003 8: 2004. Weekly samples of the predatory fauna were collected from four selected fields at lsmailia Governorate cotton fields treated by Dipel 2x and Nuclear Polyhedrosis Virus (NPV) throughout and compared with the corresponding fauna collected from insecticide treated cotton fields . Samples were undertaken from cotton fields before treated of either Bio and insecticides. Data revealed that the reliable occurrence of Dipel 2x and Nuclear Polyhedrosis Virus (NPV). Six predators species were recorded. These were Scymnus spp. order coleoptera. Coccinella undicimpunctata. (Family : Coccinellide), Paederus alferii. (Family : Starphyvnidae). one from order Hemiptora Orius spp., (Family : Anthocooidae) one from order Neuorptera Chrysopa carnea (Family : Chrysopidae) and one from order Daiptera Syrphus spp (Family : Syrphidae ). in cotton fields during the two tested seasons .The obtained results confirmed that predator densities were 2.75 times and accordingly more numerous in the Dipel 2x and Nuclear Polyhedrosis Virus (NPV) treated fields than the corresponding cotton fields treated with recommended insecticides.